Two-hybrid screening

id: two-hybrid-screening-301-17994149
title: Two-hybrid screening
text: Two-hybrid screening is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions between two proteins or a single protein and a DNA molecule, respectively. The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, c
brand slug: wiki
category slug: encyclopedia
description: Molecular biology technique
original url: https://en.wikipedia.org/wiki/Two-hybrid_screening
date created:
date modified: 2024-04-01T03:07:33Z
main entity: {"identifier":"Q1337844","url":"https://www.wikidata.org/entity/Q1337844"}
image: {"content_url":"https://upload.wikimedia.org/wikipedia/commons/6/61/Two_hybrid_assay.svg","width":709,"height":886}
fields total: 13
integrity: 15

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