Tandem affinity purification
id:
tandem-affinity-purification-301-7319899
title:
Tandem affinity purification
text:
Tandem affinity purification (TAP) is an immunoprecipitation-based purification technique for studying protein–protein interactions. The goal is to extract from a cell only the protein of interest, in complex with any other proteins it interacted with. TAP uses two types of agarose beads that bind to the protein of interest and that can be separated from the cell lysate by centrifugation, without disturbing, denaturing or contaminating the involved complexes. To enable the protein of interest to
brand slug:
wiki
category slug:
encyclopedia
description:
original url:
https://en.wikipedia.org/wiki/Tandem_affinity_purification
date created:
date modified:
2023-01-03T20:03:09Z
main entity:
{"identifier":"Q893051","url":"https://www.wikidata.org/entity/Q893051"}
image:
{"content_url":"https://upload.wikimedia.org/wikipedia/commons/e/e9/Taptag_simple.png","width":1735,"height":2047}
fields total:
13
integrity:
14