Single-molecule real-time sequencing
id:
single-molecule-real-time-sequencing-294-4567455
title:
Single-molecule real-time sequencing
text:
Single-molecule real-time (SMRT) sequencing is a parallelized single molecule DNA sequencing method. Single-molecule real-time sequencing utilizes a zero-mode waveguide (ZMW). A single DNA polymerase enzyme is affixed at the bottom of a ZMW with a single molecule of DNA as a template. The ZMW is a structure that creates an illuminated observation volume that is small enough to observe only a single nucleotide of DNA being incorporated by DNA polymerase. Each of the four DNA bases is attached to
brand slug:
wiki
category slug:
encyclopedia
description:
Method for sequencing DNA
original url:
https://en.wikipedia.org/wiki/Single-molecule_real-time_sequencing
date created:
date modified:
2023-10-25T21:50:08Z
main entity:
{"identifier":"Q13080815","url":"https://www.wikidata.org/entity/Q13080815"}
image:
fields total:
13
integrity:
14