RNase H-dependent PCR
id:
rnase-h-dependent-pcr-246-3663305
title:
RNase H-dependent PCR
text:
RNase H-dependent PCR (rhPCR) is a modification of the standard PCR technique. In rhPCR, the primers are designed with a removable amplification block on the 3’ end. Amplification of the blocked primer is dependent on the cleavage activity of a hyperthermophilic archaeal Type II RNase H enzyme during hybridization to the complementary target sequence. This RNase H enzyme possesses several useful characteristics that enhance the PCR. First, it has very little enzymatic activity at low temperature
brand slug:
wiki
category slug:
encyclopedia
description:
Laboratory technique
original url:
https://en.wikipedia.org/wiki/RNase_H-dependent_PCR
date created:
date modified:
2023-12-03T01:29:28Z
main entity:
{"identifier":"Q48998616","url":"https://www.wikidata.org/entity/Q48998616"}
image:
{"content_url":"https://upload.wikimedia.org/wikipedia/commons/6/6d/RhPCR_mechanism.tif","width":2684,"height":2489}
fields total:
13
integrity:
15