Recombinase polymerase amplification

id: recombinase-polymerase-amplification-287-12120873
title: Recombinase polymerase amplification
text: Recombinase polymerase amplification (RPA) is a single tube, isothermal alternative to the polymerase chain reaction (PCR). By adding a reverse transcriptase enzyme to an RPA reaction it can detect RNA as well as DNA, without the need for a separate step to produce cDNA,. Because it is isothermal, RPA can use much simpler equipment than PCR, which requires a thermal cycler. Operating best at temperatures of 37–42 °C and still working, albeit more slowly, at room temperature means RPA reactions c
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original url: https://en.wikipedia.org/wiki/Recombinase_polymerase_amplification
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date modified: 2023-12-03T18:00:30Z
main entity: {"identifier":"Q17077657","url":"https://www.wikidata.org/entity/Q17077657"}
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