Protein-fragment complementation assay

id: protein-fragment-complementation-assay-248-16261846
title: Protein-fragment complementation assay
text: Within the field of molecular biology, a protein-fragment complementation assay, or PCA, is a method for the identification and quantification of protein–protein interactions. In the PCA, the proteins of interest are each covalently linked to fragments of a third protein. Interaction between the bait and the prey proteins brings the fragments of the reporter protein in close proximity to allow them to form a functional reporter protein whose activity can be measured. This principle can be applie
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category slug: encyclopedia
description:
original url: https://en.wikipedia.org/wiki/Protein-fragment_complementation_assay
date created:
date modified: 2024-03-26T08:24:20Z
main entity: {"identifier":"Q753175","url":"https://www.wikidata.org/entity/Q753175"}
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fields total: 13
integrity: 13

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