Primer walking
id:
primer-walking-199-7166737
title:
Primer walking
text:
Primer walking is a technique used to clone a gene from its known closest markers. As a result, it is employed in cloning and sequencing efforts in plants, fungi, and mammals with minor alterations. This technique, also known as "directed sequencing," employs a series of Sanger sequencing reactions to either confirm the reference sequence of a known plasmid or PCR product based on the reference sequence or to discover the unknown sequence of a full plasmid or PCR product by designing primers to
brand slug:
wiki
category slug:
encyclopedia
description:
DNA sequencing method
original url:
https://en.wikipedia.org/wiki/Primer_walking
date created:
date modified:
2023-07-11T21:29:31Z
main entity:
{"identifier":"Q3817120","url":"https://www.wikidata.org/entity/Q3817120"}
image:
fields total:
13
integrity:
14