Polymerase-endonuclease amplification reaction
id:
polymerase-endonuclease-amplification-reaction-298-12183576
title:
Polymerase-endonuclease amplification reaction
text:
Polymerase-endonuclease amplification reaction (PEAR) is a DNA amplification technology for the amplification of oligonucleotides. A target oligonucleotide and a tandem repeated antisense probe are subjected to repeated cycles of denaturing, annealing, elongation and cleaving, in which thermostable DNA polymerase elongation and strand slipping generate duplex tandem repeats, and thermostable endonuclease (PspGI) cleavage releases monomeric duplex oligonucleotides. PEAR has the potential to be a
brand slug:
wiki
category slug:
encyclopedia
description:
original url:
https://en.wikipedia.org/wiki/Polymerase-endonuclease_amplification_reaction
date created:
date modified:
2021-09-14T17:54:53Z
main entity:
{"identifier":"Q7226555","url":"https://www.wikidata.org/entity/Q7226555"}
image:
{"content_url":"https://upload.wikimedia.org/wikipedia/commons/9/92/Journal_pone_0008430_g001.png","width":600,"height":442}
fields total:
13
integrity:
14