Ortho-Nitrophenyl-β-galactoside

id: ortho-nitrophenyl-galactoside-259-2705817
title: Ortho-Nitrophenyl-β-galactoside
text: ortho-Nitrophenyl-β-galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. This compound is normally colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity by means of a colorimetric assay. β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be us
brand slug: wiki
category slug: encyclopedia
description: Chemical compound
original url: https://en.wikipedia.org/wiki/Ortho-Nitrophenyl-%CE%B2-galactoside
date created:
date modified: 2024-01-31T19:40:50Z
main entity: {"identifier":"Q287775","url":"https://www.wikidata.org/entity/Q287775"}
image: {"content_url":"https://upload.wikimedia.org/wikipedia/commons/e/e8/ONPG_structure.png","width":587,"height":366}
fields total: 13
integrity: 15

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