Nick translation
id:
nick-translation-304-1181515
title:
Nick translation
text:
Nick translation, developed in 1977 by Peter Rigby and Paul Berg, is a tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in fluorescent in situ hybridization (FISH) or blotting techniques. It can also be used for radiolabeling. This process is called nick translation because the DNA to be processed is treated with DNAase to produce
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wiki
category slug:
encyclopedia
description:
original url:
https://en.wikipedia.org/wiki/Nick_translation
date created:
date modified:
2021-12-17T15:22:11Z
main entity:
{"identifier":"Q371037","url":"https://www.wikidata.org/entity/Q371037"}
image:
fields total:
13
integrity:
13