Ligation (molecular biology)

id: ligation-molecular-biology-213-1972087
title: Ligation (molecular biology)
text: Ligation is the joining of two nucleotides, or two nucleic acid fragments, into a single polymeric chain through the action of an enzyme known as a ligase. The reaction involves the formation of a phosphodiester bond between the 3'-hydroxyl terminus of one nucleotide and the 5'-phosphoryl terminus of another nucleotide, which results in the two nucleotides being linked consecutively on a single strand. Ligation works in fundamentally the same way for both DNA and RNA. A cofactor is generally inv
brand slug: wiki
category slug: encyclopedia
description: Technique for joining nucleic acid fragments
original url: https://en.wikipedia.org/wiki/Ligation_(molecular_biology)
date created: 2013-07-18T13:09:27Z
date modified: 2024-09-12T15:19:07Z
main entity: {"identifier":"Q11756706","url":"https://www.wikidata.org/entity/Q11756706"}
image: {"content_url":"https://upload.wikimedia.org/wikipedia/commons/b/bf/Ligation.svg","width":1400,"height":900}
fields total: 13
integrity: 16

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