Genome-wide CRISPR-Cas9 knockout screens

id: genome-wide-crispr-cas9-knockout-screens-235-17493447
title: Genome-wide CRISPR-Cas9 knockout screens
text: Genome-wide CRISPR-Cas9 knockout screens aim to elucidate the relationship between genotype and phenotype by ablating gene expression on a genome-wide scale and studying the resulting phenotypic alterations. The approach utilises the CRISPR-Cas9 gene editing system, coupled with libraries of single guide RNAs (sgRNAs), which are designed to target every gene in the genome. Over recent years, the genome-wide CRISPR screen has emerged as a powerful tool for performing large-scale loss-of-function
brand slug: wiki
category slug: encyclopedia
description: Research tool in genomics
original url: https://en.wikipedia.org/wiki/Genome-wide_CRISPR-Cas9_knockout_screens
date created:
date modified: 2023-12-03T23:47:07Z
main entity: {"identifier":"Q96379172","url":"https://www.wikidata.org/entity/Q96379172"}
image: {"content_url":"https://upload.wikimedia.org/wikipedia/en/a/a4/Genome-wide_CRISPR_screen_-_Overview.png","width":959,"height":540}
fields total: 13
integrity: 15

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