G-less cassette

id: g-less-cassette-322-15779450
title: G-less cassette
text: The G-less cassette transcription assay is a method used in molecular biology to determine promoter strength in vitro. The technique involves quantification of an mRNA product with the use of a plasmid. The G-less cassette is part of a pre-constructed vector, usually containing a multiple cloning site (MCS) upstream of the cassette. For this reason, promoters of interest can be inserted directly into the MCS to ultimately measure the accuracy and efficiency of a promoter in recruiting transcript
brand slug: wiki
category slug: encyclopedia
description:
original url: https://en.wikipedia.org/wiki/G-less_cassette
date created:
date modified: 2021-10-02T18:56:14Z
main entity: {"identifier":"Q18356688","url":"https://www.wikidata.org/entity/Q18356688"}
image: {"content_url":"https://upload.wikimedia.org/wikipedia/commons/0/00/Glesscassetteassay.png","width":1177,"height":1090}
fields total: 13
integrity: 14

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