Endoribonuclease-prepared siRNA
id:
endoribonuclease-prepared-sirna-287-828044
title:
Endoribonuclease-prepared siRNA
text:
esiRNA or Endoribonuclease-prepared siRNAs are a mixture of siRNA oligos resulting from cleavage of long double-stranded RNA (dsRNA) with an endoribonuclease such as Escherichia coli RNase III or Dicer. An alternative concept to the usage of chemically synthesized siRNA for RNA Interference (RNAi) is the enzymatic digestion of long double stranded RNAs in vitro. In this case a cDNA template is amplified by PCR and tagged with two bacteriophage-promoter sequences. RNA polymerase is then used to g
brand slug:
wiki
category slug:
encyclopedia
description:
original url:
https://en.wikipedia.org/wiki/Endoribonuclease-prepared_siRNA
date created:
date modified:
2023-06-04T13:49:43Z
main entity:
{"identifier":"Q5397943","url":"https://www.wikidata.org/entity/Q5397943"}
image:
fields total:
13
integrity:
13