End-sequence profiling

id: end-sequence-profiling-273-6477308
title: End-sequence profiling
text: End-sequence profiling (ESP) is a method based on sequence-tagged connectors developed to facilitate de novo genome sequencing to identify high-resolution copy number and structural aberrations such as inversions and translocations. Briefly, the target genomic DNA is isolated and partially digested with restriction enzymes into large fragments. Following size-fractionation, the fragments are cloned into plasmids to construct artificial chromosomes such as bacterial artificial chromosomes (BAC) w
brand slug: wiki
category slug: encyclopedia
description:
original url: https://en.wikipedia.org/wiki/End-sequence_profiling
date created:
date modified: 2023-12-03T00:32:20Z
main entity: {"identifier":"Q19904064","url":"https://www.wikidata.org/entity/Q19904064"}
image: {"content_url":"https://upload.wikimedia.org/wikipedia/commons/1/18/Working_flow_ESP.jpeg","width":1278,"height":818}
fields total: 13
integrity: 14

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