Diethyl pyrocarbonate
id:
diethyl-pyrocarbonate-233-8127034
title:
Diethyl pyrocarbonate
text:
Diethyl pyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase enzymes in water and on laboratory utensils. It does so by the covalent modification of histidine (most strongly), lysine, cysteine, and tyrosine residues. DEPC-treated (and therefore RNase-free) water is used in handling of RNA in the laboratory to reduce the risk of RNA being degraded by RNases. Water is usually treated with 0.1% v/v DEPC for at least 2 hours at 37 °C and t
brand slug:
wiki
category slug:
encyclopedia
description:
Chemical compound
original url:
https://en.wikipedia.org/wiki/Diethyl_pyrocarbonate
date created:
date modified:
2023-08-26T02:12:33Z
main entity:
{"identifier":"Q421385","url":"https://www.wikidata.org/entity/Q421385"}
image:
{"content_url":"https://upload.wikimedia.org/wikipedia/commons/2/22/Diethyl_pyrocarbonate3d.png","width":1600,"height":540}
fields total:
13
integrity:
15