Cassette mutagenesis
id:
cassette-mutagenesis-201-4699497
title:
Cassette mutagenesis
text:
Cassette mutagenesis is a type of site-directed mutagenesis that uses a short, double-stranded oligonucleotide sequence to replace a fragment of target DNA. It uses complementary restriction enzyme digest ends on the target DNA and gene cassette to achieve specificity. It is different from methods that use single oligonucleotide in that a single gene cassette can contain multiple mutations. Unlike many site directed mutagenesis methods, cassette mutagenesis also does not involve primer extension
brand slug:
wiki
category slug:
encyclopedia
description:
original url:
https://en.wikipedia.org/wiki/Cassette_mutagenesis
date created:
date modified:
2024-04-01T12:41:24Z
main entity:
{"identifier":"Q1670055","url":"https://www.wikidata.org/entity/Q1670055"}
image:
{"content_url":"https://upload.wikimedia.org/wikipedia/commons/d/dc/Cassette_mutagenesis.svg","width":512,"height":784}
fields total:
13
integrity:
14