Bacterial one-hybrid system
id:
bacterial-one-hybrid-system-197-3392520
title:
Bacterial one-hybrid system
text:
The bacterial one-hybrid (B1H) system is a method for identifying the sequence-specific target site of a DNA-binding domain. In this system, a given transcription factor (TF) is expressed as a fusion to a subunit of RNA polymerase. In parallel, a library of randomized oligonucleotides representing potential TF target sequences are cloned into a separate vector containing the selectable genes HIS3 and URA3. If the DNA-binding domain (bait) binds a potential DNA target site (prey) in vivo, it will
brand slug:
wiki
category slug:
encyclopedia
description:
Method for identifying the sequence-specific target site of a DNA-binding domain
original url:
https://en.wikipedia.org/wiki/Bacterial_one-hybrid_system
date created:
date modified:
2022-07-02T06:02:24Z
main entity:
{"identifier":"Q4839995","url":"https://www.wikidata.org/entity/Q4839995"}
image:
{"content_url":"https://upload.wikimedia.org/wikipedia/commons/5/56/FigureB1H.JPG","width":960,"height":720}
fields total:
13
integrity:
15