ATPase assay

id: atpase-assay-274-13599930
title: ATPase assay
text: The ATPase assay is a membrane assay that indirectly measures the activity of efflux transporters. ATP Binding Cassette or efflux transporters mediate the transport of substrates across cell membranes against a concentration gradient. ATP cleavage is tightly linked to substrate translocation, as the energy for the substrate translocation is derived from ATP hydrolysis. ATP hydrolysis yields inorganic phosphate (Pi), which can be measured by a simple colorimetric reaction. The amount of Pi libera
brand slug: wiki
category slug: encyclopedia
description: Membrane assay
original url: https://en.wikipedia.org/wiki/ATPase_assay
date created:
date modified: 2020-11-16T17:20:01Z
main entity: {"identifier":"Q4654894","url":"https://www.wikidata.org/entity/Q4654894"}
image:
fields total: 13
integrity: 14

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