5-Bromo-4-chloro-3-indolyl phosphate

id: 5-bromo-4-chloro-3-indolyl-phosphate-280-7259398
title: 5-Bromo-4-chloro-3-indolyl phosphate
text: 5-Bromo-4-chloro-3-indolyl phosphate is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. It is, for example, used in immunoblotting, in situ hybridization, and immunohistochemistry, often in combination with nitro blue tetrazolium chloride (NBT). 5-bromo-4-chloro-3-indoxyl is oxidized by atmospheric oxygen to form the blue dye 5,5′-dibromo-4,4′-dichloro-indigo. It is also oxidized by nitroblue tetrazolium (NBT), which forms an in
brand slug: wiki
category slug: encyclopedia
description: Chemical compound
original url: https://en.wikipedia.org/wiki/5-Bromo-4-chloro-3-indolyl_phosphate
date created:
date modified: 2023-08-07T09:59:20Z
main entity: {"identifier":"Q238472","url":"https://www.wikidata.org/entity/Q238472"}
image: {"content_url":"https://upload.wikimedia.org/wikipedia/commons/8/89/BCIP.svg","width":287,"height":189}
fields total: 13
integrity: 15

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