4-Fluoro-7-nitrobenzofurazan
id:
4-fluoro-7-nitrobenzofurazan-191-85558
title:
4-Fluoro-7-nitrobenzofurazan
text:
4-Fluoro-7-nitrobenzofurazan (NBD-F) is a fluorogenic, amine labeling dye that is not fluorescent itself, but covalently reacts with secondary or primary amines to form a fluorescently labeled product. It and other fluorogenic benzofurans are used for derivitization in HPLC applications. After the fluorogenic reaction, it can be detected with an excitation wavelength of 470 nm (blue) and an emission wavelength of 530 nm (green), enabling an HPLC limit of detection of 10 fmol.
brand slug:
wiki
category slug:
encyclopedia
description:
Fluorogenic amine labelling dye
original url:
https://en.wikipedia.org/wiki/4-Fluoro-7-nitrobenzofurazan
date created:
date modified:
2024-03-21T13:21:00Z
main entity:
{"identifier":"Q72475229","url":"https://www.wikidata.org/entity/Q72475229"}
image:
{"content_url":"https://upload.wikimedia.org/wikipedia/commons/2/2c/4-fluoro-7-nitrobenzofurazan_chemical_structure.svg","width":70,"height":92}
fields total:
13
integrity:
15